Application
A. Western blot analysis of Jurkat cell lysate using anti-Bcl-2 RabMab (Cat. #AJ1082a), 1:1,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab (Cat. #AJ1082a).Application
C. Flow cytometric analysis of permeabilized Jurkat cells using anti-Bcl-2 RabMAb (red)(Cat. #AJ1082a)or a rabbit IgG (negative) (green).| Product Name | Bcl-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E17 |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 26266 Da |
|---|---|
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) |
| Tissue Specificity | Expressed in a variety of tissues. |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Endoplasmic reticulum membrane; Single-pass membrane protein |
| Entrez Gene | 596 |
|---|
Application
A. Western blot analysis of Jurkat cell lysate using anti-Bcl-2 RabMab (Cat. #AJ1082a), 1:1,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab (Cat. #AJ1082a).
Application
C. Flow cytometric analysis of permeabilized Jurkat cells using anti-Bcl-2 RabMAb (red)(Cat. #AJ1082a)or a rabbit IgG (negative) (green).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
