Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (Cat#AP1303a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Bcl-2 Antibody (BH3 Domain Specific) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCIF |
| IHC:1:10~50 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BCL2 Apoptosis regulator Bcl-2 |
|---|---|
| Molecular Weight(MW) | 26266 Da |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) |
| Tissue Specificity | Expressed in a variety of tissues. |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Endoplasmic reticulum membrane; Single-pass membrane protein |
| Entrez Gene | 596 |
|---|
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (Cat#AP1303a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:10~50 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
