Application
C. Flow cytometric analysis of permeabilized K562 cells using anti-Bcl-x RabMAb (red) (Cat. #AJ1084a) or a Rabbit IgG (negative) (green).Application
B. Immunohistochemical analysis of human paraffin-embedded prostate carcinoma tissue using anti-Bcl-x RabMAb (Cat. #AJ1084a).Application
A. Western blot analysis of K562 cell lysate using anti-Bcl-x RabMAb (Cat. #AJ1084a), 1:1,000 dilution.| Product Name | Bcl-x Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E18 |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| FC:1:50 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL2L, BCLX |
|---|---|
| Alternative Names | BCL2L1 BCL2L BCLX Bcl-2-like protein 1 Apoptosis regulator Bcl-X |
| Molecular Weight(MW) | 26049 Da |
| Function | Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis |
| Tissue Specificity | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity). Cytoplasm, cytoskeleton, centrosome. Note=Mitochondrial membranes and perinuclear envelope (By similarity). Localizes to the centrosome when phosphorylated at Ser-49 |
| Entrez Gene | 598 |
|---|
Application
C. Flow cytometric analysis of permeabilized K562 cells using anti-Bcl-x RabMAb (red) (Cat. #AJ1084a) or a Rabbit IgG (negative) (green).
Application
B. Immunohistochemical analysis of human paraffin-embedded prostate carcinoma tissue using anti-Bcl-x RabMAb (Cat. #AJ1084a).
Application
A. Western blot analysis of K562 cell lysate using anti-Bcl-x RabMAb (Cat. #AJ1084a), 1:1,000 dilution.| Application Notes | FC:1:50 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
