Application
BCL2 Antibody (Center) pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. BCL2 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).| Product Name | BCL2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BCL2 Apoptosis regulator Bcl-2 |
|---|---|
| Molecular Weight(MW) | 26266 Da |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) |
| Tissue Specificity | Expressed in a variety of tissues. |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Endoplasmic reticulum membrane; Single-pass membrane protein |
| Entrez Gene | 596 |
|---|---|
| Nucleotide Accession | NP_000624.2 |
Application
BCL2 Antibody (Center) pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. BCL2 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
