Application
Western blot analysis of BCL3 Antibody (Center) in mouse liver tissue lysates (35ug/lane). BCL3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of BCL3 Antibody (Center) (Cat#AP9337c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
BCL3 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BCL3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCL3; BCL4; D19S37; B-cell lymphoma 3 protein; Proto-oncogene BCL3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL4 D19S37 |
|---|---|
| Alternative Names | BCL3 BCL4 D19S37 B-cell lymphoma 3 protein Proto-oncogene BCL3 |
| Molecular Weight(MW) | 47584 Da |
| Function | Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity) |
| Tissue Specificity | This BCL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248~277 amino acids from the Center region of human BCL3. |
| Cellular Localization | Nucleus. Cytoplasm (By similarity). Cytoplasm, perinuclear region (By similarity). Note=Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation (By similarity) |
| Entrez Gene | 602 |
|---|
Application
Western blot analysis of BCL3 Antibody (Center) in mouse liver tissue lysates (35ug/lane). BCL3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of BCL3 Antibody (Center) (Cat#AP9337c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
BCL3 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
