Application
Western blot analysis of BCL-3 in Jurkat cell lysate.| Product Name | BCL3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bcl-3Bcl-3, a proto-oncogene, is believed to be involved in certain human B cell leukemias. It encodes a protein that functions as an IkB-like molecule for native NFkB, but is specific for the p50 subunit of NFkB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| Western blot analysis (2-4 µg/ml). Per researchers feedback, the antibody also works well in Immunoprecipation (10-20 µg/ml), and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. Detects 55-60 kDa Bcl-3 of human, mouse, and rat origins. Other species have not been tested.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D19S37 BCL3 BCL4 |
|---|---|
| Target | Bcl-3 |
| Tissue Specificity | This antibody is specific for Bcl-3. |
| Cellular Localization | Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. |
Application
Western blot analysis of BCL-3 in Jurkat cell lysate.| Application Notes | Western blot analysis (2-4 µg/ml). Per researchers feedback, the antibody also works well in Immunoprecipation (10-20 µg/ml), and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. Detects 55-60 kDa Bcl-3 of human, mouse, and rat origins. Other species have not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
