Application
Western blot analysis of BCL2L1 Antibody (Center) in Y79, mouse cerebellum and brain tissue cell line lysates (35ug/lane). BCL2L1 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of BCL2L1 Antibody (Center)(Cat#AP7958c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
BCL2L1 Antibody (Center) (AP7958c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BCL2L1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | BCL2L1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCL2L1; BCL2L; BCLX; Bcl-2-like protein 1; Apoptosis regulator Bcl-X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL2L BCLX |
|---|---|
| Alternative Names | BCL2L1 BCL2L BCLX Bcl-2-like protein 1 Apoptosis regulator Bcl-X |
| Molecular Weight(MW) | 26049 Da |
| Function | Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis |
| Tissue Specificity | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity). Cytoplasm, cytoskeleton, centrosome. Note=Mitochondrial membranes and perinuclear envelope (By similarity). Localizes to the centrosome when phosphorylated at Ser-49 |
| Entrez Gene | 598 |
|---|
Application
Western blot analysis of BCL2L1 Antibody (Center) in Y79, mouse cerebellum and brain tissue cell line lysates (35ug/lane). BCL2L1 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of BCL2L1 Antibody (Center)(Cat#AP7958c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
BCL2L1 Antibody (Center) (AP7958c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BCL2L1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
