Application
A. Western blot analysis on 3T3 cell lysates using anti-phospho Bcr (pY177) RabMAb (Cat. #AJ1086a), 1:2000 dilution. The membrane was either (A) untreated (B) treated with pervanadate.| Product Name | Bcr Antibody Phospho (pY177) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCR, BCR1, D22S11, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26 |
| Modification | p-Y177 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR576(2)Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCR1 D22S11 |
|---|---|
| Alternative Names | BCR BCR1 D22S11 Breakpoint cluster region protein Renal carcinoma antigen NY-REN-26 |
| Molecular Weight(MW) | 142819 Da |
| Function | GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity |
| Tissue Specificity | A synthetic peptide corresponding to residues surrounding tyrosine 177 of human Bcr was used as immunogen. |
| Entrez Gene | 613 |
|---|
Application
A. Western blot analysis on 3T3 cell lysates using anti-phospho Bcr (pY177) RabMAb (Cat. #AJ1086a), 1:2000 dilution. The membrane was either (A) untreated (B) treated with pervanadate.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
