Application
Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.Application
BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BHLHB5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BHLHE22; BHLHB5; TNRC20; Class E basic helix-loop-helix protein 22; Class B basic helix-loop-helix protein 5; Trinucleotide repeat-containing gene 20 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB5 TNRC20 |
|---|---|
| Alternative Names | BHLHE22 BHLHB5 TNRC20 Class E basic helix-loop-helix protein 22 Class B basic helix-loop-helix protein 5 Trinucleotide repeat-containing gene 20 protein |
| Molecular Weight(MW) | 36997 Da |
| Function | Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA (By similarity) |
| Tissue Specificity | Brain-specific, with the highest expression in the cerebellum |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 27319 |
|---|
Application
Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.
Application
BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
