Application
A. Western blot analysis of Jurkat cell lysate using anti Bid RabMAb (catalog# AJ1099a).Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Bid RabMAb (Cat. #AJ1099a).| Product Name | Bid Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BID, BH3-interacting domain death agonist p11, p11 BID |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y8 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BID BH3-interacting domain death agonist p11 p11 BID |
|---|---|
| Molecular Weight(MW) | 21995 Da |
| Function | The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2 |
| Tissue Specificity | Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level) |
| Cellular Localization | Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic BH3-interacting domain death agonist p13: Mitochondrion membrane (By similarity). Note=Associated with the mitochondrial membrane (By similarity) Isoform 3: Cytoplasm. |
| Entrez Gene | 637 |
|---|
Application
A. Western blot analysis of Jurkat cell lysate using anti Bid RabMAb (catalog# AJ1099a).
Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Bid RabMAb (Cat. #AJ1099a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
