Application
BIN2 Antibody (N-term) western blot analysis in HL-60,CEM,MDA-MB435,A549 cell line lysates (15ug/lane).This demonstrates the BIN2 antibody detected the BIN2 protein (arrow).Application
BIN2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BIN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
BIN2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BIN2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BIN2; BRAP1; Bridging integrator 2; Breast cancer-associated protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRAP1 |
|---|---|
| Alternative Names | BIN2 BRAP1 Bridging integrator 2 Breast cancer-associated protein 1 |
| Molecular Weight(MW) | 61874 Da |
| Function | Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis |
| Tissue Specificity | Detected in natural killer cells (at protein level). Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues |
| Cellular Localization | Cytoplasm. Cell projection, podosome membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cell cortex. Cell projection, phagocytic cup Note=Associates with membranes enriched in phosphoinositides Detected in the actin-rich cell cortex at the leading edge of migrating cells. Detected at podosomes, at an actin-rich ring-like structure |
| Entrez Gene | 51411 |
|---|---|
| Nucleotide Accession | NP_057377.2 |
Application
BIN2 Antibody (N-term) western blot analysis in HL-60,CEM,MDA-MB435,A549 cell line lysates (15ug/lane).This demonstrates the BIN2 antibody detected the BIN2 protein (arrow).
Application
BIN2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BIN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
BIN2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
