Application
Western blot analysis of BMI1 Antibody in MCF7 cell line lysates (35ug/lane). BMI1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with BMI1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of BMI1 Antibody(Cat#AP8756a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
BMI1 Antibody flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BMI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BMI1; PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PCGF4 RNF51 |
|---|---|
| Alternative Names | BMI1 PCGF4 RNF51 Polycomb complex protein BMI-1 Polycomb group RING finger protein 4 RING finger protein 51 |
| Molecular Weight(MW) | 36949 Da |
| Function | Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 |
| Tissue Specificity | This BMI1 antibody is generated from rabbits immunized with BMI1 recombinant protein. |
| Cellular Localization | Nucleus. Cytoplasm. |
| Entrez Gene | 648 |
|---|
Application
Western blot analysis of BMI1 Antibody in MCF7 cell line lysates (35ug/lane). BMI1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with BMI1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of BMI1 Antibody(Cat#AP8756a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
BMI1 Antibody flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
