Application
BMI1 Antibody (Cat. #AM1930b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).Application
Confocal immunofluorescent analysis of BMI1 Antibody (Cat#AM1930b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | BMI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BMI1; PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 282CT3.7.6 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~250 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PCGF4 RNF51 |
|---|---|
| Alternative Names | BMI1 PCGF4 RNF51 Polycomb complex protein BMI-1 Polycomb group RING finger protein 4 RING finger protein 51 |
| Molecular Weight(MW) | 36949 Da |
| Function | Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 |
| Tissue Specificity | This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein. |
| Cellular Localization | Nucleus. Cytoplasm. |
| Entrez Gene | 648 |
|---|---|
| Nucleotide Accession | NP_005171.4 |
Application
BMI1 Antibody (Cat. #AM1930b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).
Application
Confocal immunofluorescent analysis of BMI1 Antibody (Cat#AM1930b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~250 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
