Application
Western blot analysis of anti-BMPR2 Pab (Cat. #ap2006a) in mouse heart tissue lysate. BMPR2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with BMPR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human Kidney tissue reacted with BMPR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using BMPR2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BMPR2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BMPR2; PPH1; Bone morphogenetic protein receptor type-2; Bone morphogenetic protein receptor type II |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPH1 |
|---|---|
| Alternative Names | BMPR2 PPH1 Bone morphogenetic protein receptor type-2 Bone morphogenetic protein receptor type II |
| Molecular Weight(MW) | 115201 Da |
| Function | On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs |
| Tissue Specificity | Highly expressed in heart and liver. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 659 |
|---|
Application
Western blot analysis of anti-BMPR2 Pab (Cat. #ap2006a) in mouse heart tissue lysate. BMPR2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with BMPR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human Kidney tissue reacted with BMPR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using BMPR2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
