Application
Western blot analysis of BPGM Antibody (Center) in Hela cell line lysates (35ug/lane). BPGM (arrow) was detected using the purified Pab.Application
Western blot analysis of BPGM (arrow) using rabbit polyclonal BPGM Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BPGM gene.Application
BPGM Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BPGM Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BPGM; Bisphosphoglycerate mutase; 2, 3-bisphosphoglycerate mutase, erythrocyte; 2, 3-bisphosphoglycerate synthase; 2, 3-diphosphoglycerate mutase; BPG-dependent PGAM |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BPGM Bisphosphoglycerate mutase 2 3-bisphosphoglycerate mutase erythrocyte 2 3-bisphosphoglycerate synthase 2 3-diphosphoglycerate mutase BPG-dependent PGAM |
|---|---|
| Molecular Weight(MW) | 30005 Da |
| Function | Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3- bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities |
| Tissue Specificity | Expressed in red blood cells. Expressed in non-erythroid cells of the placenta; present in the syncytiotrophoblast layer of the placental villi at the feto- maternal interface (at protein level) |
| Entrez Gene | 669 |
|---|
Application
Western blot analysis of BPGM Antibody (Center) in Hela cell line lysates (35ug/lane). BPGM (arrow) was detected using the purified Pab.
Application
Western blot analysis of BPGM (arrow) using rabbit polyclonal BPGM Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BPGM gene.
Application
BPGM Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
