Application
Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35ug/lane). BRAF (arrow) was detected using the purified Mab.Application
Confocal immunofluorescent analysis of BRAF Antibody(Cat#AM1821a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | BRAF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 125CT13.3.5 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500~16000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRAF1 RAFB1 |
|---|---|
| Alternative Names | BRAF BRAF1 RAFB1 Serine/threonine-protein kinase B-raf Proto-oncogene B-Raf p94 v-Raf murine sarcoma viral oncogene homolog B1 |
| Molecular Weight(MW) | 84437 Da |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron |
| Tissue Specificity | Brain and testis. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity) |
Application
Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35ug/lane). BRAF (arrow) was detected using the purified Mab.
Application
Confocal immunofluorescent analysis of BRAF Antibody(Cat#AM1821a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:500~16000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
