Application
Figure 1: Western blot analysis using anti-Braf monoclonal antibody against truncated recombinant Braf (1) and A431 cell lysate (2).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue(right) showing cytoplasmic localization using anti-BRAF with DAB staining.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-BRAF mAb.| Product Name | BRAF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRAF |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H12F1, 1H12G10,1F12F11C9 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRAF1 RAFB1 |
|---|---|
| Alternative Names | BRAF |
| Molecular Weight(MW) | 84437 Da |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron |
| Tissue Specificity | Brain and testis. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity) |
| Entrez Gene | 673 |
|---|---|
| Nucleotide Accession | NP_004324.2 |
Application
Figure 1: Western blot analysis using anti-Braf monoclonal antibody against truncated recombinant Braf (1) and A431 cell lysate (2).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue(right) showing cytoplasmic localization using anti-BRAF with DAB staining.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-BRAF mAb.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of BRAF expressed in E. Coli. |
