Application
Figure 1: Western blot analysis using BRAF monoclonal antibody against Hela (1), HL60 (2), HepG2(3) and NIH/3T3 (4) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-BRAF mAb.Application
Figure 3: Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using anti-BRAF mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye| Product Name | BRAF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRAF1; RAFB1; B-RAF1; FLJ95109 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H12 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRAF1 RAFB1 |
|---|---|
| Alternative Names | BRAF1 RAFB1 B-RAF1 FLJ95109 |
| Molecular Weight(MW) | 84437 Da |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron |
| Tissue Specificity | Brain and testis. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity) |
| Entrez Gene | 673 |
|---|---|
| Nucleotide Accession | NP_004324.2 |
Application
Figure 1: Western blot analysis using BRAF monoclonal antibody against Hela (1), HL60 (2), HepG2(3) and NIH/3T3 (4) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-BRAF mAb.
Application
Figure 3: Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using anti-BRAF mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human BRAF expressed in E. Coli. |
