Application
BRD2-Q185 western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BRD2 antibody detected the BRD2 protein (arrow).Application
Western blot analysis of BRD2 (arrow) using rabbit polyclonal BRD2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | BRD2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRD2; KIAA9001; RING3; Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA9001 RING3 |
|---|---|
| Alternative Names | BRD2 KIAA9001 RING3 Bromodomain-containing protein 2 O27.1.1 Really interesting new gene 3 protein |
| Molecular Weight(MW) | 88061 Da |
| Function | May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly |
| Tissue Specificity | This BRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the center region of human BRD2. |
| Cellular Localization | Nucleus (Probable). Note=Detected on chromatin and nucleosomes |
| Entrez Gene | 6046 |
|---|
Application
BRD2-Q185 western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BRD2 antibody detected the BRD2 protein (arrow).
Application
Western blot analysis of BRD2 (arrow) using rabbit polyclonal BRD2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
