Application
BRSK1 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the BRSK1 antibody detected the BRSK1 protein (arrow).| Product Name | BRSK1(Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRSK1; KIAA1811; SAD1; SADB; Serine/threonine-protein kinase BRSK1; Brain-selective kinase 1; Brain-specific serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1811 SAD1 SADB |
|---|---|
| Alternative Names | BRSK1 KIAA1811 SAD1 SADB Serine/threonine-protein kinase BRSK1 Brain-selective kinase 1 Brain-specific serine/threonine-protein kinase 1 Serine/threonine-protein kinase SAD-B Synapses of Amphids Defective homolog 1 |
| Molecular Weight(MW) | 85087 Da |
| Function | Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma- tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C |
| Tissue Specificity | Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome (By similarity). Cell junction, synapse (By similarity). Note=Localizes to synaptic vesicles in neurons (By similarity). Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage |
| Entrez Gene | 84446 |
|---|---|
| Nucleotide Accession | NP_115806.1 |
Application
BRSK1 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the BRSK1 antibody detected the BRSK1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
