Application
(LEFT)Western blot analysis of BUB1B Antibody (N-term) in Hela cell line lysates (35ug/lane).BUB1B (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BUB1B(arrow) using rabbit polyclonal BUB1B Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).| Product Name | BUB1B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; Mitotic checkpoint kinase MAD3L; Protein SSK1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BUBR1 MAD3L SSK1 |
|---|---|
| Alternative Names | BUB1B BUBR1 MAD3L SSK1 Mitotic checkpoint serine/threonine-protein kinase BUB1 beta MAD3/BUB1-related protein kinase Mitotic checkpoint kinase MAD3L Protein SSK1 |
| Molecular Weight(MW) | 119545 Da |
| Function | Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression |
| Tissue Specificity | Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index |
| Cellular Localization | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5 |
| Entrez Gene | 701 |
|---|
Application
(LEFT)Western blot analysis of BUB1B Antibody (N-term) in Hela cell line lysates (35ug/lane).BUB1B (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BUB1B(arrow) using rabbit polyclonal BUB1B Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
