Application
Western blot analysis of BUB3 antibody (Center) in HL60 cell line lysates (35ug/lane). BUB3 (arrow) was detected using the purified Pab.Application
Western blot analysis of BUB3 antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). BUB3 (arrow) was detected using the purified Pab.Application
BUB3 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BUB3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BUB3; Mitotic checkpoint protein BUB3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BUB3 Mitotic checkpoint protein BUB3 |
|---|---|
| Molecular Weight(MW) | 37155 Da |
| Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1 |
| Tissue Specificity | This BUB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196~226 amino acids from the Center region of human BUB3. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore (By similarity). Note=Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition (By similarity) |
| Entrez Gene | 9184 |
|---|
Application
Western blot analysis of BUB3 antibody (Center) in HL60 cell line lysates (35ug/lane). BUB3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of BUB3 antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). BUB3 (arrow) was detected using the purified Pab.
Application
BUB3 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
