Application
A. Western blot analysis on NIH 3T3 cell lysate using anti-Phospho-c-Jun (pS63) RabMAb (Cat. #AJ1188c), dilution 1:10,000. Cells were either untreated or treated with UV or Anisomycin.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-c-Jun (pS63) RabMAb (Cat. #AJ1188c).| Product Name | c-Jun Antibody Phospho (pS63) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JUN, Transcription factor AP-1, Proto-oncogene c-jun;V-jun avian sarcoma virus 17 oncogene homolog;p39 |
| Modification | p-S63 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y172 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JUN Transcription factor AP-1 Proto-oncogene c-jun V-jun avian sarcoma virus 17 oncogene homolog p39 |
|---|---|
| Molecular Weight(MW) | 35676 Da |
| Function | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Serine 63 of human c-Jun was used as immunogen. The antibody only detects c-Jun phosphorylated on Serine 63. Predicted to cross-react with bovine, based on sequence homology. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3725 |
|---|
Application
A. Western blot analysis on NIH 3T3 cell lysate using anti-Phospho-c-Jun (pS63) RabMAb (Cat. #AJ1188c), dilution 1:10,000. Cells were either untreated or treated with UV or Anisomycin.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-c-Jun (pS63) RabMAb (Cat. #AJ1188c).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
