Application
Western blot analysis of anti-C-rel (NFkB) Pab (AP6336a)in Jurkat (left) and Hela (right) cell line lysate. C-rel (NFkB)(arrow) was detected using the purified Pab.| Product Name | C-rel (NFkB) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | REL; Proto-oncogene c-Rel |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | REL Proto-oncogene c-Rel |
|---|---|
| Molecular Weight(MW) | 68520 Da |
| Function | Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF- kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF- kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator |
| Tissue Specificity | This C-rel (NFkB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 411~440 amino acids from the center region of human C-rel(NFkB). |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 5966 |
|---|
Application
Western blot analysis of anti-C-rel (NFkB) Pab (AP6336a)in Jurkat (left) and Hela (right) cell line lysate. C-rel (NFkB)(arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
