Application
C12orf48 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C12orf48 antibody detected the FBXO34 protein (arrow).Application
C12orf48 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C12orf48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
C12orf48 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | C12orf48 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARPBP; C12orf48; PARI; PCNA-interacting partner; PARP-1 binding protein; PARP1-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C12orf48 PARI |
|---|---|
| Alternative Names | PARPBP C12orf48 PARI PCNA-interacting partner PARP-1 binding protein PARP1-binding protein |
| Molecular Weight(MW) | 65054 Da |
| Function | Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Binds single-strand DNA and poly(A) homopolymers. Positively regulate the poly(ADP- ribosyl)ation activity of PARP1; however such function may be indirect |
| Tissue Specificity | Restricted to testis. Overexpressed in multiple cancer cells |
| Cellular Localization | Cytoplasm (By similarity). Nucleus. Note=Localizes to chromatin in response to S phase arrest but not in mitosis. Targeted to chromatin via its interaction with PCNA |
| Entrez Gene | 55010 |
|---|---|
| Nucleotide Accession | NP_060385.3 |
Application
C12orf48 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C12orf48 antibody detected the FBXO34 protein (arrow).
Application
C12orf48 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C12orf48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
C12orf48 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
