Application
Western blot analysis of C13orf3 Antibody (Center) in Hela, 293 cell line lysates (35ug/lane). C13orf3 (arrow) was detected using the purified Pab.Application
C13orf3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C13orf3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
C13orf3 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | C13orf3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SKA3; C13orf3; RAMA1; Spindle and kinetochore-associated protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C13orf3 RAMA1 |
|---|---|
| Alternative Names | SKA3 C13orf3 RAMA1 Spindle and kinetochore-associated protein 3 |
| Molecular Weight(MW) | 46359 Da |
| Function | Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization |
| Tissue Specificity | This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the Center region of human C13orf3. |
| Cellular Localization | Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner |
| Entrez Gene | 221150 |
|---|
Application
Western blot analysis of C13orf3 Antibody (Center) in Hela, 293 cell line lysates (35ug/lane). C13orf3 (arrow) was detected using the purified Pab.
Application
C13orf3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C13orf3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
C13orf3 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
