Application
Western blot analysis of C14orf133 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). C14orf133 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with C14orf133 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
C14orf133 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | C14orf133 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPE39; C14orf133; VIPAR; Spermatogenesis-defective protein 39 homolog; VPS33B-interacting protein in polarity and apical restriction |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C14orf133 SPE39 VIPAR |
|---|---|
| Alternative Names | SPE39 C14orf133 VIPAR Spermatogenesis-defective protein 39 homolog VPS33B-interacting protein in polarity and apical restriction |
| Molecular Weight(MW) | 57005 Da |
| Function | May play a role in vesicular trafficking during spermatogenesis (By similarity). Plays a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes. May play a role in epithelial polarization through stabilization of apical membrane protein content, possibly via the RAB11A-dependent apical recycling pathway. Also involved in direct or indirect transcriptional regulation of E-cadherin |
| Tissue Specificity | This C14orf133 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249~278 amino acids from the Center region of human C14orf133. |
| Cellular Localization | Cytoplasm (By similarity). Cytoplasmic vesicle (By similarity). Early endosome. Recycling endosome. Late endosome. Note=Colocalizes in clusters with VPS33B at cytoplasmic organelles (By similarity) |
| Entrez Gene | 63894 |
|---|
Application
Western blot analysis of C14orf133 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). C14orf133 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with C14orf133 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
C14orf133 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
