Application
C3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the C3 antibody detected the C3 protein (arrow).Application
Confocal immunofluorescent analysis of C3 Antibody (C-term) (Cat#AP13691b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | C3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C3; CPAMD1; Complement C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; Complement C3 beta chain; Complement C3 alpha chain; C3a anaphylatoxin; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragm |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPAMD1 |
|---|---|
| Alternative Names | C3 CPAMD1 Complement C3 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 Complement C3 beta chain Complement C3 alpha chain C3a anaphylatoxin Complement C3b alpha' chain Complement C3c alpha' chain fragment 1 Complement C3dg fragm |
| Molecular Weight(MW) | 187148 Da |
| Function | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates Acylation stimulating protein (ASP): adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for GPR77. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of GPR77 |
| Tissue Specificity | Plasma. The acylation stimulating protein (ASP) is expressed in adiopocytes and released into the plasma during both the fasting and postprandial periods |
| Cellular Localization | Secreted. |
| Entrez Gene | 718 |
|---|---|
| Nucleotide Accession | NP_000055.2 |
Application
C3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the C3 antibody detected the C3 protein (arrow).
Application
Confocal immunofluorescent analysis of C3 Antibody (C-term) (Cat#AP13691b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM228276