Application
Western blot analysis of C4orf16 Antibody (N-term) in WiDr cell line lysates (35ug/lane). C4orf16 (arrow) was detected using the purified Pab.Application
Western blot analysis of C4orf16 Antibody (N-term) in NIH-3T3 cell line lysates (35ug/lane). C4orf16 (arrow) was detected using the purified Pab.Application
C4orf16 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C4orf16 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
C4orf16 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | C4orf16 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP1AR; C4orf16; AP-1 complex-associated regulatory protein; 2c18; Adapter-related protein complex 1-associated regulatory protein; Gamma-1-adaptin brefeldin A resistance protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C4orf16 |
|---|---|
| Alternative Names | AP1AR C4orf16 AP-1 complex-associated regulatory protein 2c18 Adapter-related protein complex 1-associated regulatory protein Gamma-1-adaptin brefeldin A resistance protein |
| Molecular Weight(MW) | 34280 Da |
| Function | Necessary for AP-1 dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/Gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with AP1G1/Gamma1- adaptin attenuates the release of the AP-1 complex from membranes |
| Tissue Specificity | This C4orf16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77~107 amino acids from the N-terminal region of human C4orf16. |
| Cellular Localization | Golgi apparatus, trans-Golgi network. Late endosome. Early endosome. Note=Localizes to the juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle between the juxta-nuclear area and the cell periphery |
| Entrez Gene | 55435 |
|---|
Application
Western blot analysis of C4orf16 Antibody (N-term) in WiDr cell line lysates (35ug/lane). C4orf16 (arrow) was detected using the purified Pab.
Application
Western blot analysis of C4orf16 Antibody (N-term) in NIH-3T3 cell line lysates (35ug/lane). C4orf16 (arrow) was detected using the purified Pab.
Application
C4orf16 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C4orf16 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
C4orf16 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
