Application
Western blot analysis of CA12 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). CA12 (arrow) was detected using the purified Pab.Application
CA12 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CA12 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CA12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; Tumor antigen HOM-RCC-3.1.3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CA12 Carbonic anhydrase 12 Carbonate dehydratase XII Carbonic anhydrase XII Tumor antigen HOM-RCC-3.1.3 |
|---|---|
| Molecular Weight(MW) | 39451 Da |
| Function | Reversible hydration of carbon dioxide. |
| Tissue Specificity | Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 771 |
|---|
Application
Western blot analysis of CA12 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). CA12 (arrow) was detected using the purified Pab.
Application
CA12 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
