Application
Western blot analysis of CA5B Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CA5B (arrow) was detected using the purified Pab.Application
CA5B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CA5B Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CA5B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CA5B; Carbonic anhydrase 5B, mitochondrial; Carbonate dehydratase VB; Carbonic anhydrase VB |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CA5B Carbonic anhydrase 5B mitochondrial Carbonate dehydratase VB Carbonic anhydrase VB |
|---|---|
| Molecular Weight(MW) | 36434 Da |
| Function | Reversible hydration of carbon dioxide. |
| Tissue Specificity | Strongest expression in heart, pancreas, kidney, placenta, lung, and skeletal muscle. Not expressed in liver |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 11238 |
|---|
Application
Western blot analysis of CA5B Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CA5B (arrow) was detected using the purified Pab.
Application
CA5B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CA5B Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
