Application
Western blot analysis of CA9 antibody (Center) (Cat.# AP5000e) in Hela cell line lysates (35ug/lane). CA9 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of hela cells using CA9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Immunofluorescence analysis of CA9 Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.| Product Name | CA9 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CA9; G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; pMW1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G250 MN |
|---|---|
| Alternative Names | CA9 G250 MN Carbonic anhydrase 9 Carbonate dehydratase IX Carbonic anhydrase IX Membrane antigen MN P54/58N Renal cell carcinoma-associated antigen G250 pMW1 |
| Molecular Weight(MW) | 49698 Da |
| Function | Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia |
| Tissue Specificity | Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus |
| Entrez Gene | 768 |
|---|
Application
Western blot analysis of CA9 antibody (Center) (Cat.# AP5000e) in Hela cell line lysates (35ug/lane). CA9 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of hela cells using CA9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Immunofluorescence analysis of CA9 Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
