IHC
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human CD235a (GYPA)) Monoclonal Antibody, Unconjugated human CD235a (GYPA) Mouse mAb at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse) instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human CD235a (GYPA)) Monoclonal Antibody, Unconjugated human CD235a (GYPA) Mouse mAb at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse) instructionsand DAB staining.| Product Name | human CD235a (GYPA) Mouse mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-500 IHC:1:100-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | GYPA |
|---|---|
| Gene Synonyms | MN GPA MNS GPSAT PAS-2 CD235a GPErik HGpMiV HGpMiXI HGpSta(C) |
| Gene Full Name | glycophorin A (MNS blood group) |
| Gene Summary | Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008] |
| Alternative Names | CD235a GPA GPErik GPSAT HGpMiV HGpMiXI HGpSta(C) MN MNS PAS-2 GLPA_HUMAN GYPA MN sialoglycoprotein Sialoglycoprotein alpha GLPA_MOUSE glycophorin A (MNS blood group) glycophorin A (includes MN blood group) glycophorin A (MN blood group) glycophorinA |
| Molecular Weight(MW) | 14 kDa(Observed band size: 51 kDa) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Appears to be colocalized with SLC4A1. |
IHC
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human CD235a (GYPA)) Monoclonal Antibody, Unconjugated human CD235a (GYPA) Mouse mAb at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse) instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human CD235a (GYPA)) Monoclonal Antibody, Unconjugated human CD235a (GYPA) Mouse mAb at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse) instructionsand DAB staining.| Application Notes | WB:1:100-500 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
