Application
A. Western blot analysis on (A) Jurkat cell lysate and (B) human brain lysate using anti-CaMKIV RabMAb (Cat. #AJ1116a), dilution 1:2000.| Product Name | Calcium/Calmodulin Kinase IV (CaMKIV) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAMK4, Calcium/calmodulin-dependent protein kinase type IV, CAM kinase-GR |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2564Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAMK CAMK-GR CAMKIV |
|---|---|
| Alternative Names | CAMK4 Calcium/calmodulin-dependent protein kinase type IV CAM kinase-GR |
| Molecular Weight(MW) | 51926 Da |
| Function | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18 |
| Tissue Specificity | Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue |
| Cellular Localization | Cytoplasm. Nucleus. Note=Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity) |
| Entrez Gene | 814 |
|---|
Application
A. Western blot analysis on (A) Jurkat cell lysate and (B) human brain lysate using anti-CaMKIV RabMAb (Cat. #AJ1116a), dilution 1:2000.| Application Notes | WB:1:1000~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
