Application
Immunoblot of oxidized WT CaMKII probed with antiserum against oxidized M281/282 with increasing ratios of oxidized peptide.Application
Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized or total CaMKII and blue staining for nuclei.Application
Immunoblot of WT CaMKII and M281/282V mutant after no treatment, oxidation, or autophosphorylation probed with antibodies against total, autophosphorylated, or oxidized M281/282 CaMKII .Application
Immunofluorescent staining of heart sections from mice treated with saline, AngII, or Isoproterenol and probed for oxidized or total CaMKII. Red staining is positive for oxidized or total CaMKII, and blue staining is positive for nuclei. Mice lacking a critical subunit of NADPH oxidase (p47-/-) did not show increased levels of oxidized CaMKII in response to AngII.| Product Name | CaMKII antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody against the oxidized M281/282 form of CAMKIICalcium/calmodulin-dependent protein kinase II (CaMKII) couples increases in Ca2+ to fundamental responses in excitable cells including activation of ion channels, gene transcription and apoptosis. CaMKII is activated by angiotensin II-induced oxidation, which leads to apoptosis in cardiomyocytes. CaMKII activity is also enhanced by pro-oxidant conditions, and the oxidation of paired regulatory domain methionine residues sustains CaMKII activity in the absence of Ca2+/CaM. |
| Immunogen | CaMKII synthetic peptide with oxidized Met281/282 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| WB:1:500~1:2000 IHC-F:1:100~1:500 IHC-P:1:50~1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAMK CAMK-II CAMKG |
|---|---|
| Tissue Specificity | Mouse. Predicted to react with human based on 100% sequence identity. |
Application
Immunoblot of oxidized WT CaMKII probed with antiserum against oxidized M281/282 with increasing ratios of oxidized peptide.
Application
Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized or total CaMKII and blue staining for nuclei.
Application
Immunoblot of WT CaMKII and M281/282V mutant after no treatment, oxidation, or autophosphorylation probed with antibodies against total, autophosphorylated, or oxidized M281/282 CaMKII .
Application
Immunofluorescent staining of heart sections from mice treated with saline, AngII, or Isoproterenol and probed for oxidized or total CaMKII. Red staining is positive for oxidized or total CaMKII, and blue staining is positive for nuclei. Mice lacking a critical subunit of NADPH oxidase (p47-/-) did not show increased levels of oxidized CaMKII in response to AngII.| Application Notes | WB:1:500~1:2000 IHC-F:1:100~1:500 IHC-P:1:50~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | This product is supplied as unpurified antiserum containing 0.05% NaN3. |
