Application
A. Western blot analysis on (A) T47D, (B) fetal brain, (C) fetal spleen and (D) 293T cell lysates using anti-Calpain 1 (small subunit) RabMAb (Cat. #AJ1121a), dilution 1:2000.Application
B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 (small subunit) RabMAb (Cat. #AJ1121a).| Product Name | Calpain-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAPNS1, CAPN4, CAPNS, Calpain small subunit 1, Calpain regulatory subunit;Calcium-activated neutral proteinase small subunit |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3324 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAPN4 CAPNS |
|---|---|
| Alternative Names | CAPNS1 CAPN4 CAPNS Calpain small subunit 1 Calpain regulatory subunit Calcium-activated neutral proteinase small subunit |
| Molecular Weight(MW) | 28316 Da |
| Function | Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Calpain 1 (small subunit) was used as an immunogen. |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocates to the plasma membrane upon calcium binding (By similarity) |
| Entrez Gene | 826 |
|---|
Application
A. Western blot analysis on (A) T47D, (B) fetal brain, (C) fetal spleen and (D) 293T cell lysates using anti-Calpain 1 (small subunit) RabMAb (Cat. #AJ1121a), dilution 1:2000.
Application
B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 (small subunit) RabMAb (Cat. #AJ1121a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
