Application
Western blot analysis of Calponin-3 antibody (Center) (Cat.# AP6581c) in NIH-3T3 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.Application
Calponin-3 Antibody (Center) (Cat.# AP6581c) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Calponin-3 Antibody (Center) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Calponin-3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CNN3; Calponin-3; Calponin, acidic isoform |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CNN3 Calponin-3 Calponin acidic isoform |
|---|---|
| Molecular Weight(MW) | 36414 Da |
| Function | Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity |
| Tissue Specificity | Expressed in both non-smooth muscle tissues as well as smooth muscle tissues |
| Entrez Gene | 1266 |
|---|
Application
Western blot analysis of Calponin-3 antibody (Center) (Cat.# AP6581c) in NIH-3T3 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.
Application
Calponin-3 Antibody (Center) (Cat.# AP6581c) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Calponin-3 Antibody (Center) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
