WB
Western blot analysis using Calreticulin mouse mAb against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.WB
Western blot analysis using Calreticulin mouse mAb against Hela(1) MCF-7(2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Mouse brain(A) Mouse kidney(B) using Calreticulin mouse mAb with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Rabbit brain(A) Rabbit kidney(B) using Calreticulin mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of COS7 cells using Calreticulin mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using Calreticulin mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.FC
Flow cytometric analysis of C6 cells using Calreticulin mouse mAb (green) and negative control (red).FC
Flow cytometric analysis of NIH/3T3 cells using Calreticulin mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to Calreticulin |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide corresponding to aa (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:50-1:500 FC:1:200-1:400 |
|
| Species Reactivity | HumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CALR |
|---|---|
| Gene Synonyms | RO CRT SSA CALR1 cC1qR HEL-S-99n |
| Gene Full Name | calreticulin |
| Gene Summary | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] |
| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Cytoplasm,Cytoplasmic vesicle,Endoplasmic reticulum,Extracellular matrix,Lysosome. |
| Entrez Gene | 811 |
|---|---|
| Nucleotide Accession | NP_004334.1 |
WB
Western blot analysis using Calreticulin mouse mAb against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.
WB
Western blot analysis using Calreticulin mouse mAb against Hela(1) MCF-7(2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse brain(A) Mouse kidney(B) using Calreticulin mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Rabbit brain(A) Rabbit kidney(B) using Calreticulin mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of COS7 cells using Calreticulin mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using Calreticulin mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
FC
Flow cytometric analysis of C6 cells using Calreticulin mouse mAb (green) and negative control (red).
FC
Flow cytometric analysis of NIH/3T3 cells using Calreticulin mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:50-1:500 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
