Application
CAMK2N2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CAMK2N2 antibody detected the CAMK2N2 protein (arrow).Application
CAMK2N2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAMK2N2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of CAMK2N2 Antibody (N-term)(Cat#AP11140a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CAMK2N2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAMK2N2; Calcium/calmodulin-dependent protein kinase II inhibitor 2; CaM-KII inhibitory protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CAMK2N2 Calcium/calmodulin-dependent protein kinase II inhibitor 2 CaM-KII inhibitory protein |
|---|---|
| Molecular Weight(MW) | 8658 Da |
| Function | Potent and specific cellular inhibitor of CaM-kinase II (CAMK2). Traps Ca(2+)/calmodulin on CAMK2. May play an important role in the regulation of cell growth when overexpressed in colon adenocarcinoma LoVo cells. Traps Ca(2+)/calmodulin on CAMK2 |
| Tissue Specificity | Expressed in cell lines including hemopoietic cell lines and some tumor cell lines. Highly Expressed in stimulated dendritic cell (DC) and weakly expressed in unstimulated mature and immature DC. Highly expressed in kidney, liver, in cell lines HeLaS3, lymphoblastic leukemia MOLT-4, and Burkitt's lymphoma Raji. Moderately expressed in heart, skeletal muscle, placenta, and chronic myelogenous leukemia K-562 cells Weakly expressed in small intestine, colorectal adenocarcinoma SW480, and lung carcinoma A-549 cells |
| Cellular Localization | Nucleus. Cytoplasm, cytosol. Note=Excluded from nucleus when coexpressed with activated CAMK2 (By similarity) |
| Entrez Gene | 94032 |
|---|---|
| Nucleotide Accession | NP_150284.1 |
Application
CAMK2N2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CAMK2N2 antibody detected the CAMK2N2 protein (arrow).
Application
CAMK2N2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAMK2N2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of CAMK2N2 Antibody (N-term)(Cat#AP11140a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
