Application
A. Western blot analysis on (A) SH-SY5Y (B) human brain (C) mouse brain (D) rat brain lysate using anti-CaMKI RabMAb (Cat. #AJ1126b), dilution 1:5000.Application
B. Immunohistochemical analysis of paraffin-embedded human kidney using anti-CaMK1 RabMAb (Cat. #AJ1126b).| Product Name | CaMKI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2217Y |
| Host Species | Rabbit |
| Tested Applications | WB,IHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CAMK1 Calcium/calmodulin-dependent protein kinase type 1 CaM kinase I alpha |
|---|---|
| Molecular Weight(MW) | 41337 Da |
| Function | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF] Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser- 694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser- 498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen- stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I |
| Tissue Specificity | Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Note=Predominantly cytoplasmic (By similarity) |
| Entrez Gene | 8536 |
|---|
Application
A. Western blot analysis on (A) SH-SY5Y (B) human brain (C) mouse brain (D) rat brain lysate using anti-CaMKI RabMAb (Cat. #AJ1126b), dilution 1:5000.
Application
B. Immunohistochemical analysis of paraffin-embedded human kidney using anti-CaMK1 RabMAb (Cat. #AJ1126b).| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
