Application
Western blot analysis of CAN12 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). CAN12 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CAN12 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CAN12 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CAN12 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAPN12; Calpain-12; Calcium-activated neutral proteinase 12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CAPN12 Calpain-12 Calcium-activated neutral proteinase 12 |
|---|---|
| Molecular Weight(MW) | 81037 Da |
| Function | Calcium-regulated non-lysosomal thiol-protease (By similarity) |
| Tissue Specificity | This CAN12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52~82 amino acids from the N-terminal region of human CAN12. |
| Entrez Gene | 147968 |
|---|
Application
Western blot analysis of CAN12 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). CAN12 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CAN12 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CAN12 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
