Application
Western blot analysis of CAPN2 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). CAPN2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CAPN2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CAPN2 Antibody (C-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CAPN2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAPN2; CANPL2; Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CANPL2 |
|---|---|
| Alternative Names | CAPN2 CANPL2 Calpain-2 catalytic subunit Calcium-activated neutral proteinase 2 Calpain M-type Calpain large polypeptide L2 Calpain-2 large subunit Millimolar-calpain |
| Molecular Weight(MW) | 79995 Da |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding |
| Entrez Gene | 824 |
|---|
Application
Western blot analysis of CAPN2 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). CAPN2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CAPN2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CAPN2 Antibody (C-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
