Application
Western blot analysis of CAPZA1 antibody (Center) in SK-Br-3 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with CAPZA1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-231 cells using CAPZA1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CAPZA1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAPZA1; F-actin-capping protein subunit alpha-1; CapZ alpha-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CAPZA1 F-actin-capping protein subunit alpha-1 CapZ alpha-1 |
|---|---|
| Molecular Weight(MW) | 32923 Da |
| Function | F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments |
| Tissue Specificity | This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82~111 amino acids from the Center region of human CAPZA1. |
| Cellular Localization | Cytoplasm, cytoskeleton (By similarity). |
| Entrez Gene | 829 |
|---|
Application
Western blot analysis of CAPZA1 antibody (Center) in SK-Br-3 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with CAPZA1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-231 cells using CAPZA1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
