Application
Western blot analysis of CARD11 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). CARD11 (arrow) was detected using the purified Pab.Application
CARD11 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CARD11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CARD11 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CARD11 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CARD11; CARMA1; Caspase recruitment domain-containing protein 11; CARD-containing MAGUK protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CARMA1 |
|---|---|
| Alternative Names | CARD11 CARMA1 Caspase recruitment domain-containing protein 11 CARD-containing MAGUK protein 1 |
| Molecular Weight(MW) | 133284 Da |
| Function | Involved in the costimulatory signal essential for T- cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10 |
| Tissue Specificity | Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL- 60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells |
| Cellular Localization | Cytoplasm. Membrane raft. Note=Colocalized with DPP4 in membrane rafts |
| Entrez Gene | 84433 |
|---|
Application
Western blot analysis of CARD11 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). CARD11 (arrow) was detected using the purified Pab.
Application
CARD11 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CARD11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CARD11 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
