Application
Western blot analysis of CARKD Antibody (Center) in MCF-7 cell line lysates (35ug/lane). CARKD (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CARKD Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CARKD Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CARKD Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CARKD; ATP-dependent (S)-NAD(P)H-hydrate dehydratase; ATP-dependent NAD(P)HX dehydratase; Carbohydrate kinase domain-containing protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CARKD ATP-dependent (S)-NAD(P)H-hydrate dehydratase ATP-dependent NAD(P)HX dehydratase Carbohydrate kinase domain-containing protein |
|---|---|
| Molecular Weight(MW) | 36576 Da |
| Function | Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity) |
| Tissue Specificity | This CARKD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CARKD. |
| Cellular Localization | Mitochondrion (By similarity). |
| Entrez Gene | 55739 |
|---|
Application
Western blot analysis of CARKD Antibody (Center) in MCF-7 cell line lysates (35ug/lane). CARKD (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CARKD Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CARKD Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
