Application
Western blot analysis of CARD8 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CARD8 (arrow) was detected using the purified Pab.Application
CARD8 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CARD8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CARD8 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CARD8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CARD8; KIAA0955; NDPP1; Caspase recruitment domain-containing protein 8; Apoptotic protein NDPP1; CARD-inhibitor of NF-kappa-B-activating ligand; DACAR; Tumor up-regulated CARD-containing antagonist of CASP9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0955 NDPP1 |
|---|---|
| Alternative Names | CARD8 KIAA0955 NDPP1 Caspase recruitment domain-containing protein 8 Apoptotic protein NDPP1 CARD-inhibitor of NF-kappa-B-activating ligand DACAR Tumor up-regulated CARD-containing antagonist of CASP9 |
| Molecular Weight(MW) | 48933 Da |
| Function | Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases |
| Tissue Specificity | High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 22900 |
|---|
Application
Western blot analysis of CARD8 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CARD8 (arrow) was detected using the purified Pab.
Application
CARD8 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CARD8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CARD8 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
