Application
A. Western blot analysis of HeLa cell lysate using anti-Pro-Caspase-10 RabMab (Cat. #AJ1129a), 1,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Caspase 10 RabMab (Cat. #AJ1129a).Application
C. Caspase 10 expression in apoptotic HeLa cells detected by Immunofluorescent staining using anti-Pro-Caspase 10 RAbMab (Cat. #AJ1129a).Application
D. Flow cytometric analysis of permeabilized HeLa cells using anti-Pro-Caspase-10 RabMAb (red) (Cat. #AJ1129a)or a rabbit IgG (negative) (green).| Product Name | Caspase-10 Antibody (Pro) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP10, MCH4, Caspase-10 subunit p12, ICE-like apoptotic protease 4;Apoptotic protease Mch-4;FAS-associated death domain protein interleukin-1B-converting enzyme 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E35 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCH4 |
|---|---|
| Alternative Names | CASP10 MCH4 Caspase-10 subunit p12 ICE-like apoptotic protease 4 Apoptotic protease Mch-4 FAS-associated death domain protein interleukin-1B-converting enzyme 2 |
| Molecular Weight(MW) | 58951 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC |
| Tissue Specificity | Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon |
| Entrez Gene | 843 |
|---|
Application
A. Western blot analysis of HeLa cell lysate using anti-Pro-Caspase-10 RabMab (Cat. #AJ1129a), 1,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Caspase 10 RabMab (Cat. #AJ1129a).
Application
C. Caspase 10 expression in apoptotic HeLa cells detected by Immunofluorescent staining using anti-Pro-Caspase 10 RAbMab (Cat. #AJ1129a).
Application
D. Flow cytometric analysis of permeabilized HeLa cells using anti-Pro-Caspase-10 RabMAb (red) (Cat. #AJ1129a)or a rabbit IgG (negative) (green).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
