Application
CASQ1 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (15ug/lane).This demonstrates the CASQ1 antibody detected the CASQ1 protein (arrow).Application
CASQ1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASQ1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CASQ1 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CASQ1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASQ1; CASQ; Calsequestrin-1; Calmitin; Calsequestrin, skeletal muscle isoform |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CASQ |
|---|---|
| Alternative Names | CASQ1 CASQ Calsequestrin-1 Calmitin Calsequestrin skeletal muscle isoform |
| Molecular Weight(MW) | 45160 Da |
| Function | Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+) |
| Tissue Specificity | This CASQ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human CASQ1. |
| Cellular Localization | Sarcoplasmic reticulum lumen. Mitochondrion. Note=This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial according to PubMed:7945294 |
| Entrez Gene | 844 |
|---|---|
| Nucleotide Accession | NP_001222.2 |
Application
CASQ1 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (15ug/lane).This demonstrates the CASQ1 antibody detected the CASQ1 protein (arrow).
Application
CASQ1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASQ1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CASQ1 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
