Application
A. Western blot analysis on HeLa cell lysate using anti- Phospho-p120 Catenin (Y228) RabMAb (Cat. #AJ1138a), dilution 1:5,000-20,000. Cell lysates were (1) untreated, (2) treated with Pervanadate, or (3) treated with Phosphatase.Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Phospho-p120 Catenin (Y228) RabMAb (Cat. #AJ1138a).| Product Name | Catenin (p120) Antibody Phospho (pY228) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTNND1, KIAA0384, Catenin delta-1, p120(cas) |
| Modification | p-Y228 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E205 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0384 |
|---|---|
| Alternative Names | CTNND1 KIAA0384 Catenin delta-1 p120(cas) |
| Molecular Weight(MW) | 108170 Da |
| Function | Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins Implicated both in cell transformation by SRC and in ligand- induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage |
| Tissue Specificity | Expressed in vascular endothelium. |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Note=Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts. NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm |
| Entrez Gene | 1500 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti- Phospho-p120 Catenin (Y228) RabMAb (Cat. #AJ1138a), dilution 1:5,000-20,000. Cell lysates were (1) untreated, (2) treated with Pervanadate, or (3) treated with Phosphatase.
Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Phospho-p120 Catenin (Y228) RabMAb (Cat. #AJ1138a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
