Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Caveolin2 (pY27) RabMAb (Cat. #AJ1141b), 1:2000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Product Name | Caveolin-2 Antibody Phospho (pY27) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAV2, Caveolin-2 |
| Modification | p-Y27 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2221Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CAV2 Caveolin-2 |
|---|---|
| Molecular Weight(MW) | 18291 Da |
| Function | May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity) |
| Tissue Specificity | Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts |
| Cellular Localization | Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein Note=Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27- phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments |
| Entrez Gene | 858 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Caveolin2 (pY27) RabMAb (Cat. #AJ1141b), 1:2000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
